Free PDF Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry), by Robert K. Scopes
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Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry), by Robert K. Scopes
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New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
- Sales Rank: #813320 in Books
- Published on: 1993-11-19
- Original language: English
- Number of items: 1
- Dimensions: 9.21" h x 1.00" w x 6.14" l, 1.50 pounds
- Binding: Hardcover
- 380 pages
Most helpful customer reviews
0 of 0 people found the following review helpful.
Great Reference for Protein Purification
By Brian Barron
This book is great. It provides good, detailed biochemical explanations / theory for a variety of techniques used in protein purification, which are essential for troubleshooting tricky purifications or just getting more knowledgeable in the field.
10 of 10 people found the following review helpful.
The best book for understanding protein purification!
By A Customer
Scientists and educators alike will benefit from Robert K. Scopes
thorough and up-to-date examination of the techniques behind the science
of protein purification. Written with enough simplicity and easy
reading for the upper-level undergraduate student, but with all the
facts and formulas for hard-core theorists, Protein Purification may
well be the most concise and easy to read book of its kind on the
textbook market. A must read for those interested in any form of
protein science, anyone from the industrial biochemist to the graduate
student will benefit from Robert's full description of the most current
procedures for separating proteins by size, charge, or physical
characteristics. An in-depth discussion of procedures used from the
beginning of scientists' knowledge of the individual characteristics
of protein charge and size to the most current procedures involving dye
ligand chromatography will ensure an overall knowledge of the field.
Robert's belief that a ninety percent separation of protein is
the minimun standard for any reliable purification procedure is augmented
by his insistence that most science can be done well while watching a
budget. Definitely a textbook for the educator who has less money on
hand than the typical research scientist, I highly recommend this
book for undergraduate and graduate level biochemistry courses, as its
cost is much less than that generally charged for most college texts.
10 of 10 people found the following review helpful.
A Clear, In-depth Treatment of Biochemistry
By Gerald J. Nora
In the first chapter of Protein Purification, Scopes notes that more and more biochemical techniques are black boxed, with kits eliminating the need for an understanding of biochemical principles (e.g., "precipitate with Acme Solution B, pour off Solution B, and redissolve precipitant in Solution C"). Scopes notes that with a proper understanding of biochemistry, one can save money on kits and do more purification steps with standard lab materials and reagents. He wrote this book to help biochemistry labs make more informed decisions on protein purification rather than just defaulting on some already familiar kit or protocol.
Scopes succeeded in this purpose with flying colors: he hit a rare balance that makes this book fun to read. Each chapter is well-laid out, concetrating on a single separation technique's general practice and the theoretical foundations for that method. The basic ideas and materials are laid out, with clear, well-picked illustrations.
I was particularly happy with the mathematical treament Scopes gives: understanding the thermodynamics or physics of a process aids your intuitive understanding and makes your troubleshooting skills much more honed.
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